Crystal structures of bovine odorant-binding protein in complex with odorant molecules
نویسندگان
چکیده
منابع مشابه
An Odorant-binding Protein in Bovine Colostrum
1. General features of OBP OBP was first found in 1985 in bovine nasal mucosa as an abundant protein, which is capable of binding bell pepper odor, 2-isobutyl-3-methoxy pyridine. This protein has a hydrophobic internal cavity in the β-barrel domain, and it can bind low molecular weight (usually less than 300 Da) hydrophobic molecules in the cavity with broad specificities. Generally its affinit...
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The stability and functionality of GCC-bOBP, a monomeric triple mutant of bovine odorant binding protein, was investigated, in the presence of denaturant and in acidic pH conditions, by both protein and 1-aminoanthracene ligand fluorescence measurements, and compared to that of both bovine and porcine wild type homologues. Complete reversibility of unfolding was observed, though refolding was c...
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Odorant-binding proteins (OBPs) are lipocalins secreted in the nasal mucus of vertebrates, which convey odorants to their neuronal receptors. We compared the binding properties of a recombinant rat OBP (OBP-1F) using a set of six odorants of various chemical structures. We examined the binding properties by both fluorescent probe competition and isothermal titration calorimetry. OBP-1F affinity...
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Pheromone-sensitive sensilla trichodea of nine moth species belonging to six families and three superfamilies of Lepidoptera were immunolabelled with an antiserum against the pheromone-binding protein of Antheraea polyphemus. Strong immunolabelling of the sensillum lymph was observed in all long sensilla trichodea of A. polyphemus, A. pernyi (Saturniidae), Bombyx mori (Bombycidae) and Manduca s...
متن کاملAn odorant-binding protein facilitates odorant transfer from air to hydrophilic surroundings in the blowfly.
Chemical sense-related lipophilic ligand-binding protein (CRLBP) is an insect odorant-binding protein (OBP) found abundantly in the taste and olfactory organs of the blowfly, Phormia regina. Through computational construction, a three-dimensional molecular model of a CRLBP indicated good fitting to a fluorescent ligand, 7-hydroxycoumarin (7-HC), in its ligand-binding pocket. By showing that the...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2004
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.2004.04315.x